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This International Standard specifies a high-performance liquid chromatographic (HPLC) method for the determination of the carbadox content in premixtures and animal feeding stuffs.

The method is applicable to animal feeding stuffs with a mass fraction of carbadox of 0,5 mg/kg (limit of quantification) to 100 mg/kg, and to premixtures with a mass fraction of carbadox up to 10 %.

The lower limit of detection is 0,1 mg/kg.